BIODECOLOURIZATION OF TEXTILE EFFLUENT USING BIOSURFACTANT PRODUCED BY PSEUDOMONAS AERUGINOSA AND BACILLUS SUBTILIS ISOLATED FROM ENGINE OIL CONTAMINATED SOIL
Abstract
ABSTRACT
Biosurfactants can be characterized according to their chemical composition, molecular weight, physicochemical properties, mode of action and microbial origin. Microbial decolorization and degradation is an environment friendly and cost-competitive alternative to chemical decomposition processes. This study therefore was carried to with the aim to produce and characterized biosurfactants produced by Pseudomonas and Bacillus species for the decolorization of textile effluents. Bacteria from oil polluted soil was isolated by standard spread plate technique using nutrient agar. Extraction of biosurfactant was done using the acid precipitation method and the weight of the biosurfactant was determined. GCMS and FTIR analysis of the biosurfactant were carried out according to standard method, and the extent of decolourization caused by the biosurfactant was estimated and expressed as percentage (%) decolourization. The result indicated that the dry weight of biosurfactant produced by Pseudomonas aeruginosa was estimated to be (1.2±0.03), while Bacillus subtilis was 1.38±0.02. The GC/MS analysis of the biosurfactant produced by Pseudomonas aeruginosa was a rhamnolipid and detected 12 compounds present in the biosurfactant while the biosurfactant produced by Bacillus subtilis was glycolipid and the constituents present in the extract where 17 in numbers. Biosurfactants produced by Bacillus subtilis decolorized textile effluent by 67.7%; on the other hand, biosurfactant produced by Pseudomonas aeruginosa decolorized textile effluent by 13.7%. Bacillus subtilis and Pseudomonas aeruginosa proved to be good biosurfactant producers and hence good in decolorization of textile dyes and effluents.
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