SERUM BIOCHEMICAL AND HISTOLOGICAL ANALYSIS FOLLOWING SUB-ACUTE DOSING OF ETHYLACETATE FRACTION OF NELSONIA CAMPESTRIS IN RATS

Abstract

The present study assessed the effect of sub-acute administration of ethyl acetate fraction of Nelsonia campestris on biochemical and liver histopathology of male rats. The sub-acute toxicity study was conducted by oral administration of the extracts at daily doses of 150, 300 and 600 mg/kg body weight to rats for 28 days. The extract caused a significant (p< 0.05) increase in alkaline phosphatase (ALP), aspartate aminotransferase (AST). There was a slight increase in total protein at both 150 and 300 mg/kg in the parameters with ethyl acetate fraction.  The serum alanine transaminase (ALT), bilirubin compared favorably (p> 0.05) with the control. The histopathology examination showed no remarkable changes in liver morphology of the rats treated with extract at 100 & 300 mg/kg body weight.  However, the liver of animals treated with higher doses of ethyl acetate fraction (600 mg/kg) showed noticeable cellular alterations. In conclusion, ethyl acetate fraction shows significant alteration in some biochemical parameters of liver integrity.